Oxford Nanopore MinION Workshop

Oxford Nanopore experts will provide attendees with a comprehensive data analysis overview followed by practical hands-on experience running different analysis workflows.

Day 1 – Monday 25 March

Venue: UQ Centre for Clinical Research, The Royal Brisbane & Women’s Hospital Campus

Oxford Nanopore experts, Alina Ham PhD, (Technical Applications Specialist) and Androo Markham PhD, (Field Applications Specialist) will provide attendees with a nanopore sequencing overview and case study discussion.

A nanopore sequencing demonstration as well as a rapid sequencing control experiment will allow each participant to gain practical hands-on experience of running the MinION/GridION.

The workshop will cover the following:

• Comprehensive technology overview
• Library preparation in groups
• Library loading practice
• Running the MinION/GridION
• Introduction to basecalling and EPI2ME analysis workflow

Day 2 – Tuesday 26 March

Venue: Clinical Skills Development Service, Royal Brisbane & Women’s Hospital Campus

Oxford Nanopore experts will provide attendees with a comprehensive data analysis overview followed by practical hands-on experience running different analysis workflows.

This workshop will give participants experience with real-time analysis tools as well as in-depth analysis workflows with command line tools.

The workshop will cover the following:

• In depth overview of EPI2ME for real-time end-to-end analysis
• Command line tutorial
• Guppy (command, parameters and folder structure)
• Overview of fast5 file format
• Quality and read length distribution plot and filtering
• Mapping to a reference
• De novo assembly and consensus correction